PHONE

+86(027) 5111 3188

Share to:

GB12192 Anti -Vimentin Mouse Monoclonal Antibody

Immunogen: KLH conjugated Synthetic peptide corresponding to Mouse vimentin 
Isotype: IgG
Purity: Affinity purification
ul:
  • GB12192
  • Servicebio

Description

Product Information


Description

Vimentin mouse monoclonal

Protein full name

Vimentin

Synonyms

VIM, CTRCT30, HEL113, vimentin

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse vimentin

Isotype

IgG

Predicted MW.

57 kDa

Observed MW.

57 kDa

Purity

Affinity purification

Subcellular location

Cytoplasm, Nucleus

Uniprot ID

P08670, P20152, P31000




Applications


Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1:1000-1: 2000

HeLa, HepG2, SW480,

PC12, lung

IHC

Human

1: 500-1: 1000

lung cancer,

colon, stomach,

stomach cancer

IF

Mouse

1: 500-1: 1000

NIH/ 3T3




Background



Vimentin is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells. IF proteins are found in all metazoan cells as well as bacteria. IF, along with tubulin-based microtubules and actin-based microfilaments, comprises the cytoskeleton. All IF proteins are expressed in a highly developmentally-regulated fashion; vimentin is the major cytoskeletal component of mesenchymal cells. Because of this, vimentin is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both normal development and metastatic progression.




Images



2.jpg

Western blot analysis of Vimentin (GB12192) at dilution of 1: 1000.

Lane 1: HeLa cell lysate

Lane 2: MCF7 cell lysate

Lane 3: U20S cell lysate

Lane 4: NIH/3T3 cell lysate

Lane 5: PC12 cell lysate

Lane 6: Mouse lung tissue lysate


image.png

Immunofluorescence analysis of mouse NIH/ 3T3 cells using Vimentin (GB12139) at dilution of 1: 1000

A: DAPI nuclear stain ( blue )

B: Anti- Vimentin ( red )

C: Merge

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Vimentin (GB12192) at dilution of 1: 1000

Immunohistochemistry analysis of paraffin-embedded human colon using Vimentin (GB12192) at dilution of 1: 1000

Immunohistochemistry analysis of paraffin-embedded human stomach using Vimentin (GB12192) at dilution of 1: 1000

Immunohistochemistry analysis of paraffin-embedded human stomach cancer using Vimentin (GB12192) at dilution of 1: 1000




Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).

Contact us

Quick Links

Product Category

Get In Touch

 5th Floor, 22 Building, Biopark, Gaoxin 2nd Road No. 388, East Lake High-Tech Developing Zone, Wuhan, Hubei, China 430079
 +86(027) 5111 3188
Copyright © 2021  Wuhan Servicebio Technology Co., Ltd.