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GB12839 Anti -ATG3 Mouse Monoclonal Antibody

Immunogen: KLH conjugated Synthetic peptide corresponding to Mouse ATG3
Isotype: IgG
Purity: Affinity purification
ul:
  • GB12839
  • Servicebio

Description

Product Information


Description

ATG3 mouse monoclonal

Protein full name

Ubiquitin-like-conjugating enzyme ATG3

Synonyms

Autophagy-related protein 3, Protein PC3-96, APG3-like, hApg3, ATG3, APG3, APG3L

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse ATG3

Isotype

IgG

Purity

Affinity purification

Predicted MW.

36 kDa

Observed MW.

40 kDa

Uniprot ID

Q9NT62, Q9CPX6, Q6AZ50




Applications


Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 500-1: 1000

heart, muscle, kidney




Background

ATG3 (APG3) is a widely expressed conjugating enzyme for APG8 lipidation, an essential step for the initiation of autophagy. Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. Autophagy is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG3 functions as an E2-like enzyme during the initial stages of autophagosome formation by catalyzing the formation of the Atg8-phosphatidylethanolamine (Atg8-PE) conjugate, which is critical for autophagy.




Images


图片 1.png


Western blot analysis of ATG3 (GB12839)

at dilution of 1: 1000

Lane 1: K562 cell lysate

Lane 2: THP-1 cell lysate

Lane 3: HeLa cell lysate

Lane 4: Mouse heart tissue lysate

Lane 5: Mouse muscle tissue lysate

Lane 6: Mouse kidney tissue lysate

Lane 7: Rat heart tissue lysate

Lane 8: Rat muscle tissue lysate

Lane 9: Rat kidney tissue lysate




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).

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