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Anti -AKT1 (phospho S473) Rabbit pAb for WB IHC IF ICC ELISA

specification:50uL
  • GB13012-5
  • Servicebio
  • Servicebio

Description

Product Information

 

Description

AKT1 (phospho S473) rabbit polyclonal

Protein full name

RAC-alpha serine/threonine-protein kinase

Synonyms

Protein kinase B, PKB, Protein kinase B alpha, PKB alpha, Proto-oncogene c-Akt, RAC-PK-alpha, AKT1, PKB, RAC

Immunogen

A synthesized peptide derived from human AKT1 around the phosphorylation site of Thr308.

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cell membrane, Cytoplasm, Nucleus

Predicted MW.

56 kDa

Observed MW.

56-60 kDa

Uniprot ID

P31749, P31750, P47196

 


 

Applications

 

Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 500-1: 2000

brain

IHC

Human, Mouse, Rat

1: 50-1: 200

ovary, mammary cancer, liver cancer, pancreatic cancer, gastric cancer,

IF/ICC

Human, Mouse, Rat

1: 100-1: 500

 

ELISA

Human, Mouse, Rat

1: 20000-1: 40000

 

 


 

Background

 

The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1.

 


 

Images

 

   
   

 


 

Storage

 

Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.

 

NOTE: This product is intended for research only.

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