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Anti -P53 Mouse MAb for IHC IF Cancer Research

Specification:50uL
  • GB13029-3
  • Servicebio
  • Servicebio

Description

Product Information


Description

P53 mouse monoclonal

Protein full name

Cellular tumor antigen p53

Synonyms

TP53, BCC7, LFS1, P53, TRP53, tumor protein p53

Immunogen

KLH conjugated Synthetic peptide corresponding to Human P53

Isotype

IgG

Purity

Affinity purification

Subcellular location

Nucleus, Endoplasmic reticulum, Mitochondrion

Uniprot ID

P04637




Applications


applications

species

dilution

Positive tissue

IHC

Human

1: 100-1: 200

lung cancer

IF

Human

1: 100-1: 200

colon cancer




Background



Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression.




Images



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Immunohistochemistry analysis of paraffin-embedded human lung cancer using P53 (GB13029-3) at dilution of 1:200.


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Immunofluorescence analysis of paraffin-embedded human colon cancer using P53 (GB13029-3) at dilution of 1:200.





Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE: This product is intended for research only.

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