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Anti -Histone H3 Mouse MAb For Western Histone Related

Specification:50uL
  • GB13102-1
  • Servicebio

Description

Product Information



Description

Histone H3 mouse monoclonal

Protein full name

Histone H3

Immunogen

KLH conjugated Synthetic peptide corresponding to human Histone H3

Isotype IgG
Purity Affinity purification

Predicted MW.

17 kDa

Observed MW.

17 kDa
Uniprot ID

P68431,Q71DI3,P84243




Applications



Applications

WB

Species

Human,Mouse,Rat, chicken,guinea pig, pig,zebrafish,dog,rabbit

Dilution

1:10000-1:30000

Positive tissue

general




Background



Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells.[1][2]Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Histone proteins are highly post-translationally modified however Histone H3 is the most extensively modified of the five histones. The term "Histone H3" alone is purposely ambiguous in that it does not distinguish between sequence variants or modification state. Histone H3 is an important protein in the emerging field of epigenetics, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes.




Images



blob.png

Western blot analysis of Histone H3 (GB13102-1) at dilution of 1:10000.

Lane 1: Hela cell lysate

Lane 2: HepG2 cell lysate

Lane 3: A549 cell lysate

Lane 4: HUVEN cell lysate

Lane 5: Jurkat cell lysate

Lane 6: NIH/3T3 cell lysate

Lane 7: RAW264.7 cell lysate

Lane 8: H9C2 cell lysate

B13102-1.png

Western blot analysis of Histone H3 (GB13102-1) at dilution of 1:10000.

Lane 1:Mouse mixed tissue lysate

Lane 2: Rat mixed tissue lysate

Lane 3: Chicken mixed tissue lysate

Lane 4: Guinea pig mixed tissue lysate

Lane 5: Pig mixed tissue lysate

Lane 6: zebrafish mixed tissue lysate

Lane 7: Dog mixed tissue lysate

Lane 8: Rabbit mixed tissue lysate




Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE: This product is intended for research only.

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