PHONE

+86(027) 5111 3188

Share to:

Anti -IKB Alpha Rabbit PAb For Western Blot Lab Research Antibody

Specification:100uL
  • GB111509
  • Servicebio
  • Servicebio

Description

Product Information


Description

IKB alpha rabbit polyclonal

Protein full name

NF-kappa-B inhibitor alpha

Synonyms

NFKBIA, IKBA, MAD-3, NFKBI,NFKB inhibitor alpha

Immunogen

KLH conjugated Synthetic peptide corresponding to Rat IKB alpha.

Isotype

IgG

Purity

Affinity purification

Predicted MW.

36 kDa

Observed MW.

36 kDa

Uniprot ID

P25963, Q9Z1E3, Q63746




Applications


applications

species

dilution

Positive tissue

WB

Human, Mouse, Rat

1: 500-1: 1000

spleen, skin, lung




Background


Three major forms of IKB like molecules have been identified and each is characterised by multiple copies of ankyrin repeats. IKB alpha and IKB beta appear to be the major regulatory forms of IKB in most cells. These proteins interact with p65 or cRel containing forms of NFkB and block nuclear import by masking the nuclear localisation sequences of NFkB. The activation of NFkB involves the inducible phosphorylation and subsequent degradation of IKB. Immunoblotting easily detects the hyperphosphorylated forms of IKB alpha, but not phosphorylated IKB beta. Interestingly, IKB alpha and IKB beta mediate different NFkB responses. IkB alpha appears to control more transient activation of NFkB in response to an inducer, while IKB beta controls a persistent response. Bcl3 interacts with p50 and p52 containing forms of NFkB, but rather than being an inhibitor it appears to function to stimulate transcription. The degradation of IKB is confirmed by immunoblotting.




Images



1532931770670736.png

Western blot analysis of IKB alpha (GB13212-1) at dilution of 1:1000.

Lane 1: HeLa cell lysate

Lane 2: HepG2 cell lysate

Lane 3: A431 cell lysate

Lane 4: Mouse spleen tissue lysate

Lane 5: Mouse skin tissue lysate

Lane 6: Mouse lung tissue lysate

Lane 7: Rat spleen tissue lysate

Lane 8: Rat skin tissue lysate

Lane 9: Rat lung tissue lysate




Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE: This product is intended for research only.

downLoadImgSlice 10Slice 12Slice 13

Contact us

Quick Links

Product Category

Get In Touch

 5th Floor, 22 Building, Biopark, Gaoxin 2nd Road No. 388, East Lake High-Tech Developing Zone, Wuhan, Hubei, China 430079
 +86(027) 5111 3188
Copyright © 2021  Wuhan Servicebio Technology Co., Ltd.