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Anti -CREB Mouse MAb For Western Blot Monoclonal Antibody

Specification:50uL
  • GB13236-1
  • Servicebio
  • Servicebio

Description

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Product Information


Description

CREB mouse monoclonal

Protein full name

Cyclic AMP-responsive element-binding protein 1

Synonyms

CREB1, CREB, CREB-1, cAMP responsive element binding protein 1

Immunogen

KLH conjugated Synthetic peptide corresponding to Human CREB-1.

Isotype

IgG

Purity

Affinity purification

Predicted MW.

37 kDa

Observed MW.

37 kDa

Uniprot ID

P16220, Q01147, P15337




Applications


applications

species

dilution

Positive tissue

WB

Human, Mouse, Rat

1: 500-1: 1000

spleen, lung, brain




Background



Cyclic AMP Response Element Binding protein (CREB) is a basic / leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB is dependent upon the phosphorylation of serine 133. Phosphorylation occurs via p44 / 42 MAP kinase and p90RSK and also via p38 MAP kinase and MSK 1. Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in the region of serine 133. CREB appears to play an important role in learning and memory. CREB knock out mice show diminished learning ability.




Images



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Western blot analysis of CREB (GB13236-1) at dilution of 1: 1000.

Lane 1: HeLa cell lysate

Lane 2: HepG2 cell lysate

Lane 3: NIH3T3 cell lysate

Lane 4: Mouse spleen tissue lysate

Lane 5: Mouse lung tissue lysate

Lane 6: Mouse brain tissue lysate

Lane 7: Rat spleen tissue lysate

Lane 8: Rat lung tissue lysate

Lane 9: Rat brain tissue lysate




Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE: This product is intended for research only.


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