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GB11553 Anti -Synaptophysin Rabbit pAb

Immunogen: KLH conjugated Synthetic peptide corresponding to Mouse Synaptophysin
Isotype: IgG
Purity: Affinity purification
ul:
  • GB11553
  • Servicebio

Description

Product Information

 

 

Description Synaptophysin rabbit polyclonal
Protein full name Synaptophysin

Synonyms

SYP, MRX96,MRXsynaptophysin
Immunogen KLH conjugated Synthetic peptide corresponding to Mouse Synaptophysin
Isotype IgG
Purity Affinity purification

Predicted MW.

34 kDa

Observed MW.

38 kDa
Subcellular location Cell membrane, Cytoplasm
Uniprot ID P08247, Q62277, P07825

 


 

Applications

 

Applications Species Dilution Positive Tissue
WB Human,
Mouse, Rat
1:10000 brain, cerebral cortex, cerebellum,
IHC Human,
Mouse, Rat
1:1000-1:2000 brain
IF Mouse, Rat 1:1000-1:2000 pancreas, brain, spinal cord

 


 

Background

 

 

Synaptophysin, also known as the major synaptic vesicle protein p38, is a protein that in humans is encoded by the SYP gene. The protein is a synaptic vesicle glycoprotein with four transmembrane domains weighing 38kDa. It is present in neuroendocrine cells and in virtually all neurons in the brain and spinal cord that participate in synaptic transmission. It acts as a marker for neuroendocrine tumors, and its ubiquity at the synapse has led to the use of synaptophysin immunostaining for quantification of synapses.

             


 

Images

 

 

blob.png  

Western blot analysis of Synaptophysin (GB11553) at dilution of 1:10000.

Lane 1:Human glioma tissue lysate

Lane 2:Human brain tissue lysate

Lane 3:Mouse brain tissue lysate

Lane 4:Mouse cerebral cortex tissue lysate

Lane 5:Mouse cerebellum tissue lysate

Lane 6:Rat brain tissue lysate

Lane 7:Rat cerebral cortex tissue lysate

Lane 8:Rat cerebellum tissue lysate

 

     
blob.png  

Immunohistochemistry analysis of paraffin-embedded human brain tumor using Synaptophysin (GB11553) at dilution of 1:1000

     
blob.png  

Immunohistochemistry analysis of paraffin-embedded mouse brain using Synaptophysin (GB11553) at dilution of 1:2000

     
blob.png  

Immunohistochemistry analysis of paraffin-embedded rat hippocampus using Synaptophysin (GB11553) at dilution of 1:1000

     

  Immunofluorescent analysis of paraformaldehyde-fixed mouse pancreas using SYP(GB11553) at dilution of 1:1000
 
     

  Immunofluorescent analysis of paraformaldehyde-fixed rat brain using SYP(GB11553) at dilution of 1:1000
 
     

  Immunofluorescent analysis of paraformaldehyde-fixed rat spinal cord using SYP(GB11553) at dilution of 1:1000
 
     
     

 


 

Storage

 

 

Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. 

 


 

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).

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