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Anti -GAD65 Mouse MAb for Western Blot Immunoblotting

Specification:100ul
  • GB12562
  • Servicebio
  • Servicebio

Description

Product Information

 

 

Description

GAD65 mouse monoclonal

Protein full name

Glutamate decarboxylase 2

Synonyms

GAD2, GAD65, glutamate decarboxylase 2, 65 kDa glutamic acid decarboxylase, DCE 2, Glutamic Acid Decarboxylase 2; Glutamic Acid Decarboxylase 65.

Immunogen

Recombinant protein corresponding to Mouse GAD65

Isotype

IgG

Purity

Affinity purification

Predicted MW.

65 kDa

Observed MW.

65 kDa

Subcellular location

Cell membrane, Cytoplasm

Uniprot ID

Q05683, P48320

 


 

Applications

 

 

Applications

WB

IHC

IF

Species

Mouse, Rat

Mouse, Rat

Mouse, Rat

Dilution

1: 500-1: 1000

1: 1000-1: 2000

1: 500-1: 1000

Positive tissue

brain, cerebellum, hippocampus

brain

brain

 


 

Background

 

 

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein.

             


 

Images

 

 

image.png  

Western blot analysis of GAD65 (GB12562) at dilution of 1: 2000.

Lane 1: Human brain tissue lysate

Lane 2: Mouse brain tissue lysate

Lane 3: Mouse cerebral cortex tissue lysate

Lane 4: Mouse hippocampus tissue lysate

Lane 5: Mouse cerebellum tissue lysate

Lane 6: Rat brain tissue lysate

Lane 7: Rat cerebral cortex tissue lysate

Lane 8: Rat hippocampus tissue lysate

Lane 9: Rat cerebellum tissue lysate

     
image.png  

Immunohistochemistry analysis of paraffin-embedded mouse brain using GAD65 (GB12562) at dilution of 1: 1000

     
image.png  

Immunohistochemistry analysis of paraffin-embedded mouse brain using GAD65 (GB12562) at dilution of 1: 1000

Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).

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