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Anti -EIF2S1 Mouse mAb for Human Mouse Rat Immunoblotting

Specification:100ul
  • GB12544
  • Servicebio
  • Servicebio

Description

Product Information

 

 

Description

EIF2S1 mouse monoclonal

Protein full name

Eukaryotic translation initiation factor 2 subunit 1

Synonyms

EIF2S1, EIF-2, EIF-2A, EIF-2alpha, EIF2, EIF2A, eukaryotic translation initiation factor 2 subunit alpha

Immunogen

Recombinant protein corresponding to Mouse EIF2S1

Isotype

IgG

Purity

Affinity purification

Predicted MW.

36 kDa

Observed MW.

38 kDa

Subcellular location

Cytoplasmic granule

Uniprot ID

P05198, P68101, Q6ZWX6

 


 

Applications

 

 

Applications

WB

IHC

IF

Species

Human, Mouse, Rat

Mouse, Rat

Mouse, Rat

Dilution

1: 500-1: 1000

1: 4000-1: 8000

1: 2000-1: 4000

Positive tissue

colon, brain

brain

brain

 


 

Background

 

Eukaryotic translation initiation factor 2 subunit 1 (eIF2α) is a protein that in humans is encoded by the EIF2S1 gene. The protein encoded by this gene is the alpha (α) subunit of the translation initiation factor eIF2 complex which catalyzes an early regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA (Met-tRNAiMet) to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (α, 36 kD, this article), beta (β, 38 kD), and gamma (γ, 52 kD). The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2α.

             


 

Images

 

 

image.png  

Western blot analysis of EIF2S1 (GB12544) at dilution of 1: 1000.

Lane 1: Hela cell lysate

Lane 2: HepG2 cell lysate

Lane 3: A549 cell lysate

Lane 4: NIH/3T3 cell lysate

Lane 5: PC12 cell lysate

Lane 6: Mouse colon tissue lysate

Lane 7: Mouse brain tissue lysate

Lane 8: Rat colon tissue lysate

Lane 9: Rat brain tissue lysate

     
image.png  

Immunohistochemistry analysis of paraffin-embedded mouse brain using EIF2S1 (GB12544) at dilution of 1: 6000

     
image.png  

Immunohistochemistry analysis of paraffin-embedded rat brain using EIF2S1 (GB12544) at dilution of 1: 6000

Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

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