PHONE

+86(027) 5111 3188

Share to:

Anti -Vimentin Mouse MAb for Western Immunoblotting antibody

Specification:100ul
  • GB12192
  • Servicebio
  • Servicebio

Description

Product Information

 

 

Description

Vimentin mouse monoclonal

Protein full name

Vimentin

Synonyms

VIM, CTRCT30, HEL113, vimentin

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse vimentin 

Isotype IgG
Purity Affinity purification

Predicted MW.

57 kDa

Observed MW.

57 kDa

Subcellular location

Cytoplasm

Uniprot ID

P08670P20152P31000

 


 

Applications

 

 

Applications

WB

IHC

IF

Species

Human, Mouse, Rat

Human

Mouse

Dilution

1:1000-1: 2000

1: 500-1: 1000

1: 500-1: 1000

Positive tissue

Hela, HepG2, SW480, PC12, lung

lung cancer, colon, stomach,stomach
cancer

NIH/ 3T3

 


 

Background

 

Vimentin is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells. IF proteins are found in all metazoan cells as well as bacteria. IF, along with tubulin-based  microtubules  and actin-based microfilaments, comprises the cytoskeleton. All IF proteins are expressed in a highly developmentally-regulated fashion; vimentin is the major cytoskeletal component of  mesenchymal cells. Because of this, vimentin is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both normal development and metastatic progression.        

     


 

Images

 

 

2.jpg  

Western blot analysis of Vimentin (GB12192) at dilution of 1:1000.

Lane 1: Hela cell lysate

Lane 2: MCF7 cell lysate

Lane 3: U20S cell lysate

Lane 4: NIH/3T3 cell lysate

Lane 5: PC12 cell lysate

Lane 6: Mouse lung tissue lysate

     
image.png  

Immunofluorescence analysis of mouse NIH/ 3T3 cells using Vimentin (GB12139) at dilution of 1: 1000

A: DAPI nuclear stain ( blue )

B: Anti- Vimentin ( red )

C: Merge

 


 

Storage

 

 

Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. 

 


 

NOTE: This product is intended for research only.

downLoadImgSlice 12Slice 13Slice 10 


Contact us

Quick Links

Product Category

Get In Touch

 5th Floor, 22 Building, Biopark, Gaoxin 2nd Road No. 388, East Lake High-Tech Developing Zone, Wuhan, Hubei, China 430079
 +86(027) 5111 3188
Copyright © 2021  Wuhan Servicebio Technology Co., Ltd.