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Anti -beta III Tubulin Mouse MAb for Mouse Immunofluorescence Human Western Blot IHC

Specification:100ul
  • GB12139
  • Servicebio
  • Servicebio

Description

Product Information

 

 

Description

beta III Tubulin mouse monoclonal

Protein full name

Tubulin beta-3 chain

Synonyms

TUBB3, CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, beta-4, tubulin beta 3 class III

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse β3- tubulin

Isotype IgG
Purity Affinity purification

Predicted MW.

55 kDa

Observed MW.

55 kDa

Subcellular location

Cytoplasm

Uniprot ID

Q9ERD7Q4QRB4

 


 

Applications

 

 

Applications

WB

IF

Species

Human, Mouse, Rat

Mouse

Dilution

1: 1000-1: 2000

1: 500-1: 1000

Positive tissue

brain, glioma, cortex, hippocampus

Neuro-2a

 


 

Background

 

 

Cysteine residues in class III β-tubulin are actively involved in regulating ligand interactions and microtubule formation. Proteomic analysis has revealed that many factors bound to these cysteine residues are involved in the oxidative stress and glucose deprivation response. This is particularly interesting in light of the fact that class III β-tubulin first appears in the phylogenetic tree when life emerged from the seas and cells were exposed to atmospheric oxygen.        

     


 

Images

 

 

1上传-内页.jpg  

Western blot analysis of β3- tubulin (GB12139) at dilution of 1: 1000

Lane 1: U87 cell lysate

Lane 2: Human brain tissue lysate

Lane 3: Human glioma tissue lysate

Lane 4: Mouse brain tissue lysate

Lane 5: Mouse cortex tissue lysate

Lane 6: Mouse hippocampus tissue lysate

Lane 7: Rat brain tissue lysate

Lane 8: Rat cortex tissue lysate

Lane 9: Rat hippocampus tissue lysate

     
image.png  

Immunofluorescence analysis of human Neuro-2a cells using β3- tubulin (GB12139) at dilution of 1: 1000

A: DAPI nuclear stain ( blue )

B: Anti-eGFP ( red )

C: Merge

Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

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