Cysteine residues in class III β-tubulin are actively involved in regulating ligand interactions and microtubule formation. Proteomic analysis has revealed that many factors bound to these cysteine residues are involved in the oxidative stress and glucose deprivation response. This is particularly interesting in light of the fact that class III β-tubulin first appears in the phylogenetic tree when life emerged from the seas and cells were exposed to atmospheric oxygen.
Western blot analysis of β3- tubulin (GB12139) at dilution of 1: 1000
Lane 1: U87 cell lysate
Lane 2: Human brain tissue lysate
Lane 3: Human glioma tissue lysate
Lane 4: Mouse brain tissue lysate
Lane 5: Mouse cortex tissue lysate
Lane 6: Mouse hippocampus tissue lysate
Lane 7: Rat brain tissue lysate
Lane 8: Rat cortex tissue lysate
Lane 9: Rat hippocampus tissue lysate
Immunofluorescence analysis of human Neuro-2a cells using β3- tubulin (GB12139) at dilution of 1: 1000
A: DAPI nuclear stain ( blue )
B: Anti-eGFP ( red )
Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.