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Anti -Heme Oxygenase 1 Mouse mAb for WB IHC IF

Specification:100ul
  • GB12104
  • Servicebio
  • Servicebio

Description

Product Information

 

 

Description

Heme Oxygenase 1 Mouse monoclonal

Protein full name

Heme oxygenase 1

Synonyms

HMOX1, HMOX1D, HO-1, HSP32, bK286B10, heme oxygenase 1

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse HMOX1

Isotype

IgG

Purity

Affinity purification

Predicted MW.

33 kDa

Observed MW.

33 kDa

Subcellular location

Microsome, Endoplasmic reticulum membrane

Uniprot ID

P14901

 


 

Applications

 

 

Applications

WB

IHC

IF

Species

Mouse, Rat

Mouse, Rat

Mouse, Rat

Dilution

1: 500-1: 1000

1: 1000-1: 3000

1: 500-1: 1000

Positive tissue

spleen, liver, kidney

spleen

spleen

 


 

Background

 

 

Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.

             


 

Images

 

 

image.png  

Western blot analysis of HMOX1 (GB11821) at dilution of 1: 1000

Lane 1: Raw264.7 cell lysate

Lane 2: Mouse spleen tissue lysate

Lane 3: Mouse liver tissue lysate

Lane 4: Mouse kidney tissue lysate

Lane 5: Mouse spleen tissue lysate

     
image.png  

Immunohistochemistry analysis of paraffin-embedded mouse spleen using HMOX1 (GB11821) at dilution of 1: 1500

     
image.png  

Immunohistochemistry analysis of paraffin-embedded rat spleen using HMOX1 (GB11821) at dilution of 1: 1500

Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).

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