Product Information
| Description | BrdU mouse monoclonal |
| Protein full name | Bromodeoxyuridine |
| Synonyms | Bromodeoxyuridine, Bromodeoxyuridine, 5-Bromo-2′-deoxyuridine; 5-BrdU, Proliferation Marker; 5-Bromo-2-deoxyUridine |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse BrdU |
| Isotype | IgG |
| Purity | Affinity purification |
| Subcellular location | Nuclear |
| CAS: | 59-14-3 |
Applications
| Applications | Species | Dilution | Positive Tissue |
| IHC | Human, Mouse, Rat | 1: 100-1: 500 | Jejunum |
| IF | Human, Mouse, Rat | 1: 100-1: 500 | Hela |
Background
Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. It can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU is a toxic and mutagenic substance. It triggers cell death, the formation of teratomas, alters DNA stability, lengthens the cell cycle, and has mitogenic, transcriptional and translational effects on cells that incorporate it.
Images
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Immunofluorescence analysis of Hela cells（treated with 0.03mg/ml BrdU for 40 min )using BrdU (GB12051) at dilution of 1:100 |
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Immunofluorescence analysis of Hela cells（treated with 0.03mg/ml BrdU for 2 h )using BrdU (GB12051) at dilution of 1:100 |
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Immunohistochemistry analysis of paraffin-embedded mouse jejunum(intraperitoneal injection BrdU every 2 hours for 4 times,the mouse were killed after 24 hours and the jejunum were fixed with 4%PFA)using BrdU (GB12051) at dilution of 1:300
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Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE: This product is intended for research only.
