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Anti -Brdu Mouse mAb

Cat. No. GB12051

Brand:Servicebio

Price:230.00

Specification: 100μl

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Product Information

 

Description BrdU mouse monoclonal
Protein full name Bromodeoxyuridine
Synonyms Bromodeoxyuridine, Bromodeoxyuridine,
5-Bromo-2′-deoxyuridine; 5-BrdU, Proliferation
Marker; 5-Bromo-2-deoxyUridine
Immunogen KLH conjugated Synthetic peptide corresponding to Mouse BrdU
Isotype IgG
Purity Affinity purification
Subcellular location Nuclear
CAS: 59-14-3

 


 

Applications

 

Applications Species Dilution Positive Tissue
IHC Human, Mouse, Rat 1: 100-1: 500 Jejunum
IF Human, Mouse, Rat 1: 100-1: 500 Hela
 

 


 

Background

 

 

Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. It can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU is a toxic and mutagenic substance. It triggers cell death, the formation of teratomas, alters DNA stability, lengthens the cell cycle, and has mitogenic, transcriptional and translational effects on cells that incorporate it. 

     


 

Images

 

 

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Immunofluorescence analysis of Hela cells(treated with 0.03mg/ml BrdU for 40 min )using BrdU (GB12051) at dilution of 1:100

   
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Immunofluorescence analysis of Hela cells(treated with 0.03mg/ml BrdU for 2 h )using BrdU (GB12051) at dilution of 1:100

   
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Immunohistochemistry analysis of paraffin-embedded mouse jejunum(intraperitoneal injection BrdU every 2 hours for 4 times,the mouse were killed after 24 hours and the jejunum were fixed with 4%PFA)using BrdU (GB12051) at dilution of 1:300

 

 


 

Storage

 

 

Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. 

 


 

NOTE: This product is intended for research only.