Product Information
| Description |
beta Actin mouse monoclonal |
| Protein full name | Actin, cytoplasmic 1 |
|
Synonyms |
ACTB, BRWS1, PS1TP5BP1, Beta-actin, actin beta, Beta Cytoskeletal Actin |
| Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse β-Actin |
| Isotype | IgG |
| Purity | Affinity purification |
| Predicted MW. |
42 kDa |
| Observed MW. | 45 kDa |
| Subcellular location | Cytoplasm |
| Uniprot ID | P60709, P60710, P60711 |
Applications
| Applications | WB |
IHC, IF |
|
| Species |
Human, Rat, Mouse |
Human, Rat, Mouse |
|
| Dilution |
1:1000-1:2000 |
1:400-1:2000 | |
| Positive tissue |
Hela, Raw264.7, liver, brain |
Liver, lung, stomach |
Background
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
Images
 |
Western blot analysis of β-Actin (GB12001) at dilution of 1:1000. Lane 1: Hela cell lysate Lane 2: Raw264.7 cell lysate Lane 3: C6 cell lysate Lane 4: HCT116 tumor tissue lysate Lane 5: HepG2 tumor tissue lysate Lane 6: Mouse liver tissue lysate Lane 7: Mouse brain tissue lysate Lane 8: Rat liver tissue lysate Lane 9: Rat brain tissue lysate |
 |
Immunohistochemistry analysis of paraffin-embedded human liver using β-Actin(GB12001) at dilution of 1:1300 |
 |
Immunohistochemistry analysis of paraffin-embedded mouse ovary using β-Actin(GB12001) at dilution of 1:400 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE: This product is intended for research only.