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Anti -beta Actin Mouse MAb for IHC Western Blot Loading Control

Specification:100ul
  • GB12001
  • Servicebio
  • Servicebio

Description

Product Information

 

 

Description

beta Actin mouse monoclonal

Protein full name Actin, cytoplasmic 1

Synonyms

ACTB, BRWS1, PS1TP5BP1, Beta-actin, actin beta, Beta Cytoskeletal Actin
Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse β-Actin

Isotype IgG
Purity Affinity purification
Predicted MW.

42 kDa

Observed MW. 45 kDa
Subcellular location Cytoplasm
Uniprot ID P60709, P60710, P60711
 

 


 

Applications

 

 

Applications WB

IHC, IF

 
Species

Human, Rat, Mouse

Human, Rat, Mouse

 
Dilution

1:1000-1:2000

1:400-1:2000  
Positive tissue

Hela, Raw264.7, liver, brain

Liver, lung, stomach

 

 


 

Background

 

 

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.

   


 

Images

 

 

image.png

Western blot analysis of β-Actin (GB12001) at dilution of 1:1000.

Lane 1: Hela cell lysate

Lane 2: Raw264.7 cell lysate

Lane 3: C6 cell lysate

Lane 4: HCT116 tumor tissue lysate

Lane 5: HepG2 tumor tissue lysate

Lane 6: Mouse liver tissue lysate

Lane 7: Mouse brain tissue lysate

Lane 8: Rat liver tissue lysate

Lane 9: Rat brain tissue lysate

   
image.png

Immunohistochemistry analysis of paraffin-embedded human liver using β-Actin(GB12001) at dilution of 1:1300

   
image.png

Immunohistochemistry analysis of paraffin-embedded mouse ovary using β-Actin(GB12001) at dilution of 1:400

 


 

Storage

 

 

Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.

 


 

NOTE: This product is intended for research only.

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