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What can PCR plate be used for?
This PCR plate, constructed from a single rigid piece of polypropylene, is compatible with PCR systems and thermal cyclers. PCR plates can reduce waste from smaller experiments by using the easy-to-tear dual side tabs by separating. White or clear PCR plates? Standard or low profile PCR plates? Skirted or non-skirted PCR plates? These are the questions you will meet when you are going to choose an appropriate PCR plate.
l What are the types of PCR plates?
l How to choose a right PCR plates for your experiments?
l What can PCR plates be used for?
What are the types of PCR plate?
White PCR plates direct significantly more signal back to the detector than traditional clear PCR plates. The improved signal reflection ensures that even the lowest levels of fluorescence are detected. White well walls of PCR plates also stop signal from passing through to the cycler block where it can be inconsistently reflected or absorbed, leading to increase noise levels in detected fluorescence. White PCR plates increases sensitivity, reduces variability and increases the signal-to-noise ratio.
Clear PCR plates are able to verify pipetting steps right after each pipetting action, as you can see the reagent added into the well as intended.
Standard-profile wells of PCR plates are relative to the low-profile plates and fit in most classical thermal cyclers and real-time PCR detection systems. These PCR plates reduce risk-splashing caused cross-over contamination, minimizing the risk of well-to-well cross contamination.
Low-profile wells of PCR plates are the newer design that reduces the potential for condensate formation and offers advantages for light capture in fluorescence assays, low-volume reactions, and fast PCR.
Semi-skirted PCR plates are more warp-resistant because of the rigidity provided by the skirt on the outer perimeter of the plate. These PCR plates also allow for labeling or barcoding.
Non-skirted PCR plates can be readily cut into sectional sizes as required by user.
How to choose a right PCR plates for your experiment?
The skirts of PCR plates provide mechanical strength to be able to operate easily. There are several types of PCR plates, that are, non-skirted, semi-skirted, full-skirted, and fast or low-capacity profile semi-skirted.
Non-skirted PCR plate is the simplest form. Non-skirted PCR plate is easy to distinguish because it has no skirt but only the edge of the PCR plate. Non-skirted PCR plate is suitable for most PCR instruments and timed quantitative PCR instruments.
The semi-skirted PCR plate provides strong mechanical strength and is easy to operate.
The full-skirted PCR plate has higher mechanical strength and is very suitable for robotic platforms or automated workflows. The full-skirted PCR plate is specially designed for instruments with raised thermal cycling modules. Both semi-skirted and full-skirted PCR plates have barcodes for easy recording and tracking.
Fast or low-profile semi-skirted PCR plates have a smaller volume capacity because the height of the wells of PCR plates is lower than that of standard 96-well PCR plates. These reaction plates are suitable for fast or low-profile PCR instrument modules.
What can PCR plate be used for?
PCR plate is valuable in a number of laboratory and clinical techniques, including DNA fingerprinting, detection of bacteria or viruses, and diagnosis of genetic disorders. Once amplified, the DNA produced by PCR plate can be used in many different laboratory procedures.
The specificity and sensitivity of PCR plates is highly useful for the diagnosis of various diseases in humans, including diagnosis of inherited disorders, viral diseases, and bacterial diseases. PCR plate is a real boon as it provides direct information of DNA.
PCR plate is employed in the prenatal diagnosis of inherited diseases by using chorionic villus samples or cells from amniocentesis. PCR plate is used for the detection of bacterial infection.
As the PCR plate is much simpler and quicker to amplify the DNA, it is conveniently used for sequencing. Single-strands of DNA are required. The advantage with PCR plate is that the primers need not have complementary sequences for the target DNA.
Learn more about PCR reaction plate can know the types of PCR reaction plate and the applications of PCR reaction plate. Wuhan Servicebio Technology Co., Ltd. offers a range of high-quality PCR reaction plate that can be used in a variety of contexts, from labs and hospitals to school biology classes. We also have many products about Pipette Tips and Tissue Homogenizer, If you have any questions, you can contact us immediately.