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Silver Glycine Staining Kit for Nerve Fiber Staining Reagent

800 ml+150 ml+250 ml   RT in the dark

  • G1052-500T
  • Servicebio

Product Description

Principle

 

Axon in nerve fiber is argyrophilic. In this staining method, sections are firstly treated with acid formaldehyde solution, then react with glycine-silver solution to precipitate silver ion in axon. Finally reduce silver ion to black element silver by reductant. This kit applies to paraffin sections and frozen-slides, usually used in studying neuron fibril pathological changes in Alzheimer's disease and non-Alzheimer’s disease, detecting the degree and extent of degeneration of peripheral nerve in leprosy, and differentiating neurofibroma (positive) from neurinoma (negative).   

 

Package content

 

Code

Name

Size

Preservation

G1052-1

Reductant

800 ml

RT,  in the dark

G1052-2

Glycine-silver solution

150 ml

RT,  in the dark

G1052-3

Acid formaldehyde solution

250 ml

RT,  in the dark

 

Preservation

Valid in 3 months when stored at room temperature and kept in the dark.

 

Procedure

1 Deparaffinize and rehydrate to distilled water. Wash clearly in distilled.

2 Rinse in acid formaldehyde solution for 5 min, wash three times in distilled water.

3 Rinse in preheated (37℃) glycine-silver solution for 3-5 min.

4 Remove the residual glycine-silver solution. Rinse sections in preheated (45℃) reductant I for several seconds and immediately shift to reductant II (preheated at 45℃) to react for several seconds. Wash in distilled water briefly. Treat with 5odium thiosulfate solution (5%), then wash three times in distilled water. 

5 Dehydrate in 3 changes of pure ethanol, 5 min each. Rinse in butanol for 5 min. clear in 2 changes of xylene, 5 min each. Dry briefly then mount with resin mounting medium.

6 Microscopy determination and collect images for analysis.

 

Results

 

Neuron axon, neuron fiber, neurofibrillary tangles, senile plaques and dendrite are shown black in gold yellow background.

 

Notes

1 Be sure to wash slides with distilled water when treat with glycine-silver solution.

2 Be quickly in the reduction in step 4. For the sake of good result, one section at a time is recommended.

3 All the solutions should be prevent from light. Glycine-silver can be reused for 3-5 times within a day, but use fresh reductant.

G1052-500T-G1052-500T=Slice 11Slice 12Slice 10

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