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Nissl body, also known as Nissl substance, is a large granular body located in cytoplasm of neurons. The Nissl body major contains ribosome and rough endoplasmic reticulum, is the site of protein synthesis. Distribution of Nissl body is a demonstrating neuron injury or not. This stain is commonly used for identifying the basic neuronal structure. This stain method is based on toluidine blue staining.
Nissl stain solution
Valid in 12 months at room temperature.
1. Deparaffinize and rehydrate to distilled water
2. Stain in Nissl stain solution for 10 min
3. Differentiate in 95% alcohol (proper differentiate extent for brain tissue is that Nissl body show deep blue, while background is light blue or colorless ), wash in tap water
4. Dry in air and mount with resin mounting medium
Nissl bodies are shown with deep blue, nucleus are light blue and background is colorless.