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Nissl Body Staining Reagent for Nerve Cell

  • G1036-100ML

Product Description


Nissl body, also known as Nissl substance, is a large granular body located in cytoplasm of neurons. The Nissl body major contains ribosome and rough endoplasmic reticulum, is the site of protein synthesis. Distribution of Nissl body is a demonstrating neuron injury or not. This stain is commonly used for identifying the basic neuronal structure. This stain method is based on toluidine blue staining.


Package content 





Nissl stain solution

100 ml


Valid in 12 months at room temperature.



1. Deparaffinize and rehydrate to distilled water

2. Stain in Nissl stain solution for 10 min 

3. Differentiate in 95% alcohol (proper differentiate extent for brain tissue is that Nissl body show deep blue, while background is light blue or colorless ), wash in tap water

4. Dry in air and mount with resin mounting medium



Nissl bodies are shown with deep blue, nucleus are light blue and background is colorless.


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