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Mycoplasma Elimination Reagent Plus 10ML 100× For Cell Culture Mycoplasma Inhibitor

Specification:10ML
  • G4007-10ML
  • Servicebio
  • Servicebio

Product Description

G4007-10ml

Introduction:

This product is used to remove Mycoplasma cells. It is a combination reagent of quinolones, tetracyclines and macrolides. It inhibits DNA replication and protein synthesis of Mycoplasma respectively, thereby effectively removing Mycoplasma cells. This product can completely remove the mycoplasma from infected cells within 5-7 days. After replacing the normal medium, the cells are in good condition and there is no re-positive phenomenon in continuous multi-generation culture.

 

Storage and transportation

Transport with wet ice; store at -20°C to avoid repeated freezing and thawing. The validity period is 12 months.

 

Composition:

Component

G4007-10ML

Mycoplasma Elimination Reagent Plus(100×)

10×1 mL

Instruction Manual

1pcs

Steps

 

1. Take 1 mL of Mycoplasma Elimination Reagent Plus (100×) and add it to 100 mL of cell culture medium (with serum, without double antibodies), mix well to obtain a complete medium containing Mycoplasma Elimination Reagent Plus;

 

2. When the mycoplasma-infected cells are passaged or changed, replace the medium containing Mycoplasma Elimination Reagent Plus for routine cell culture;

 

3. After culturing for 5 days, inoculate part of the cells into a 6-well plate (or other culturing utensils), and replace with normal complete medium. The remaining cells continue to be cultured with the medium containing Mycoplasma Elimination Reagent Plus;

 

4. The cells in the 6-well plate are cultured for 2-3 days with normal complete medium, and when the confluence of the cells reaches 90% or more, the mycoplasma detection is performed (G1900, G1901, G1902 are recommended);

 

5. According to the test results, perform the following operations:

 

a) The result is positive: continue to repeat steps 3-4 until the test result is negative.

 

b) The result is negative: Replace the cell group containing Mycoplasma Elimination Reagent Plus medium with a normal complete medium, culture for a period of time for mycoplasma detection, the retest result is negative, which means that the elimination is complete.

 

Precautions

 

1. This product has been tested for removal in a variety of mycoplasma-infected cell lines, but different cell lines have different sensitivity to the reagent. If the cytotoxicity is too large or the removal effect is not good, the product concentration can be adjusted or extended appropriately. For cleaning time, other scavengers can also be used (G4008 is recommended).

 

2. There are many ways to infect cells with Mycoplasma. It is recommended to add mycoplasma prevention reagents in daily culture (G4009 is recommended).

 

3. Cells that have been determined to be mycoplasma contaminated, try to use a separate ultra-clean table during operation to avoid cross-contamination.

 

4. For your safety and health, please wear lab coats and disposable gloves for operation.

Item name

Mycoplasma Elimination Reagent(100×)

Mycoplasma Elimination Reagent Plus(100×)

Mycoplasma Elimination Reagent(SweMyco-1+2)(100×)

Item No.

G4006

G4007

G4008

Composition

Quinolones, Tetracyclines

Quinolones, Tetracyclines ,Macrolides

Quinolones, Tetracyclines ,Macrolides

Relative Toxicity

Medium

High

Low

Cleaning Period

10 Days

5-7 Days

7 Days

Handling

Easy

Easy

Complicated

 

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