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α-MEM without nucleosides Solution for Cell Culture Medium 199 Media

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Cat. NO.:G4555-500ML

Volume: 500mL

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  • G4555-500ML
  • Servicebio

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Name

Cat No.

Size

α-MEM, no nucleosides

G4555-500ML

500 mL

Product description

MEM (Minimum Essential Medium) is one of the most commonly used basal medium for supporting the growth of many suspension and adherent mammalian cells. α-MEM is a modification of MEM that contains non-essential amino acids (including L-Alanine, L-Glutamic acid, L-Asparagine, L-Aspartic acid, L-Proline, L-Serine, and Glycine), sodium pyruvate, lipoic acid, vitamin B12, biotin and ascorbic acid. α-MEM can be used with a variety of suspension and adherent mammalian cells. α-MEM is avaiable without nucleosides for use as a selection medium for DG44 and other DHFR-negative cells. This product is made with Earles salts, includes non-essential amino acids, no addition of ribonucleosides and deoxyribonucleosides.

α-MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). α-MEM uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

All raw material components used for Servicebio cell culture media products are screened through using strict quality control testing. Servicebio cell culture media are manufactured at a cGMP-compliant facility using water for injection, filtered through final 0.1 μm sterile filtration and aseptic filled in a Hundred-stage FFU. The filter integrity tests are performed before and after filtration. Each lot of cell culture media are subjected to sterility test, pH, osmolality, endotoxin and cell culture test.

The major character of this MEM is as follows:

l With 5.5 mM Glucose, 2.0 mM L-glutamine, 1.0 mM sodium pyruvate, non-essential amino acid (NEAA), phenol red.

l Without ribonucleosides, deoxyribonucleosides ,HEPES.

The complete formulation is available.

Storage

Storage conditions: 2℃ to 8℃, protect from light

Shipping conditions: Ambient

Shelf life: 12 months from date of manufacture


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