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In histopathology, the tissue must be fixed in time and effectively before the subsequent embedding and sectioning can be completed. The role of tissue fixation is to maintain the inherent morphology and structure of tissues and cells, prevent bacterial corrosion and autolysis of tissue cells, preserve the inherent substances in cells, coagulate intracellular proteins, and minimize or terminate endogenous and exogenous The enzyme reaction keeps the cells or tissues basically the same as the substances in life, and can also harden the tissues and facilitate the cutting of the tissues. For some infectious specimens, it can prevent the spread of diseases; keep the general specimens. Finally, fixation can also enhance the effect of dyeing.
The main component of the in-situ hybridization fixative (animal) of this product is 4% paraformaldehyde, with 0.1 M phosphate buffer as the solvent, pH 7.0-7.5@25°C, treated with DEPC, effectively inactivating RNase in the solution, for special use For fixation, samples that require subsequent nucleic acid in situ hybridization detection.
|Cat. No.||Product description||Volume|
|G1113||ISH Fixative solution(animal)||500 mL|
Stored at room temperature, valid for 12 months.
Cut the plant into 2mm thickness and put the plant tissue into the ISH Fixative solution at room temperature by tweezers or pipettes. The ratio of the fixative solution to the tissue is greater than 10:1. Fixed 12h-24h at room temperature. If cannot cut it into 2mm³ at one time, you can also cut it into a slightly larger tissue block first, and then fix it while cutting it into 2mm³ size. Tender tissue can be reduced the time according to the size of the material.
1. After the tissue is removed in vivo, it should be put into the fixative solution as soon as possible, and the interval time should not exceed 30min. To prevent the degradation of RNA in tissues.
2. The volume of the tissue should be small, preferably no more than 2mm³. Because it takes time for the fixative solution to penetrate, if the tissue is too large, the inside of the tissue will not be well fixed.
3. The sampling position should be accurate, and the mechanical damage should be small, the anatomical instruments should be sharp, and the operation should be soft, to avoided the pulling, contusion and extrusion.