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Haematoxylin is used to stain nucleus. Except for nucleus, the cytoplasm, collagen and fiber of tissue slides may also be stained in nonspecific. This haematoxylin differentiate solution is used to selectively decolorize the over-stained nucleus and nonspecific staining area. The differentiate process is necessary to increase the contrast and resolution.
Haematoxylin differentiate solution
Valid in 6 months at room temperature.
1、 Deparaffinize sections, 2 changes of xylene, 10 minutes each. Dehydrate in 95% alcohol and 85% alcohol respectively, 5 minute each. Wash in running tap water for 1-2 min.
2、 Stain in hematoxylin solution (G1004) for 3-5 minutes, wash in tap water for 1 minute to 2 minutes
3、 Rinse in haematoxylin differentiate solution for a few seconds
4、 Rinse in bluing solution (G1040) for a few seconds, wash in tap water for 1-2 min.
5、 Carry out other routine process such as other stain, or dehydrate. Then followed by clear and mount, microscopy determination.
1、 The differentiate solution can be reused for several times. But the over-use may affect the process time and efficiency. The role of differentiate is increasing contrast of nucleus and cytoplasm. Manage the time is important, and should be adjusted according to tissue type and slide thickness. If over-differentiate and result in pale nucleus, re-stain slides is allowed. Likewise if differentiate is inadequate and result in deep-staining nucleus, one more time for differentiate is allowed, too.
2、 Please use the reagent within valid period. The staining kit should be stored avoiding extremely high or low temperature and kept in dark condition.
3、 For your safety and health, please wear a lab coat and wear disposable gloves.