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Ham's F-12 Nutrient Mixture is designed on the basis of Ham's F-10 Nutrient Mixture and was originally used for serum-free culture of CHO cells. Ham's F-12 is often used as the basic culture medium for serum-free culture. It is especially suitable for single-cell culture and clonal culture when the serum content is low. After serum is added, it is also widely used in the culture of tumor cells and primary cells, into rat hepatocytes. , Rat prostate epithelial cells, chondrocytes, rat myoblasts, chicken embryo cells, etc. In addition, Ham's F-12 and DMEM can be mixed in equal volume to obtain a more nutritious DMEM/F-12 medium.
This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, and without protein or growth factors. Add serum or serum-free additives according to the cell type.
This product is sterilized by 0.1 μm filter membrane, pH 7.0-7.4, contains 10 mM D-glucose, contains 1.0 mM L-glutamine and 1.0 mM sodium BT, contains phenolRed indicator, without HEPES buffer system.
Storage and transportation
Wet ice transportation; storage at 2-8°C, valid for at least one year.
1. This product has been filtered sterilization. When using it, should pay attention to aseptic operation to avoid contamination.
2. In order to maintain the best use effect, do not freeze and thaw.
3. This product is only used for lab research, not for diagnosis and treatment.