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G3410-1000U Recombinant HRV 3C Protease with His-tag

Specification: 1000ul
Usage: Remove MBP, GST, HIS or other tagged proteins from fusion proteins.
Storage and Transportation: Transport with wet ice; store at -20, valid for 12 months.
u:
  • G3410-1000U
  • Servicebio

Product Description

Overview

Recombinant HRV 3C Protease (Human Rhinovirus 3C Protease,Prescission, Human Rhinovirus type 14 3C Protease, a commonly used cysteine Protease, is a recombinant expression of His-tagged Human Rhinovirus type 14 3C Protease in Escherichia coli.It can specifically recognize the sequence L-E-V-L-F-Q-G-P (Leu-Glu-Val-Leu-Phe-Gly-Gly-Pro) or the core pentapeptide sequence L-F-Q-G-P (Leu-Phe-Gly-Gly-Pro) at low temperature.The enzyme digestion was performed between Q (Gln) and G-P (Gly-Pro) amino acid residues.The enzyme had strong specificity and high activity, and it still had high activity even at 4℃.

Application: It is often used to remove MBP, GST, HIS or other tagged proteins from fusion proteins.The protease itself carries the HIS label and binds to the nickel column.

Source: Human Rhinovirus type 14 3C Protease, recombinant expression of Escherichia coli, containing His label.

Definition of enzyme activity: 100 μg of substrate (protein containing HRV 3C Protease digestion site) can be cut more than 90% of the required enzyme activity unit within 16h under the condition of 4℃, pH 7.5.

Inactivation or inhibition: 100mM ZnCl2, 4mM AEBSF and 100μm Chymostatin all inhibit the activity of HRV 3C Protease.

Purity and concentration: SDS-PAGE purity ≥95%, 2 U/μL.

Recombinant HRV 3C Protease Storage solution:50 mM Tris-HCl,150 mM NaCl,1 mM EDTA,1 mM DTT,50% (v/v) glycerol,pH 8.0.

10×HRV 3C Buffer:500 mM Tris-HCl,1.5 M NaCl,10 mM EDTA,10 mM DTT,pH 7.5.


Component Number

Component

G3410-1000U

G3410-1

Recombinant HRV 3C Protease (His-tag) (2 U/μL)

500 μL

G3410-2

10×HRV 3C Buffer

3×1 mL

G3410-3

Control Protein

100 μL

Product Usage


1.Portenase digestion system recommendation

Component

Volume

10×HRV 3C Buffer

5 μL

Interest protein

200 μg

Recombinant HRV 3C Protease (His-tag) (2U/μL)

1 μL

ddH2O

To 50 μL

2. The reaction system was placed at 4℃ for overnight reaction or 16h;

3. 10μL sample of digestion product was taken for SDS-PAGE detection to evaluate the amount of digestion and reaction conditions.


Notes


1. Enzyme products should be placed in an ice box or ice bath, and stored at -20℃ immediately after use. Separate storage is recommended.

2. For your safety and health, please wear a lab coat and disposable gloves to operate.


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