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G2013-100ML 100ml Reduced 5*Protein Loading Buffer

Specifiction: 100ml
Application: Electrophoresis loading of conventional SDS-PAGE protein samples
Transportation and Storage: Dry ice transportation;Store at -20℃, effective for 12 months.
  • G2013-100ML
  • Servicebio

Product Description


The protein loading buffer of this product is a 5 times concentrated protein loading buffer with bromophenol blue dye.It is used for electrophoresis loading of conventional SDS-PAGE protein samples.The main ingredients of this product include Tris-HCl buffer, glycerin, SDS, bromophenol blue and a small amount of reducing agent DTT. 

Product name

Cat. No.


5×Protein loading buffer (reduced)


1 mL


100 mL

Product Usage




5× Protein loading buffer (reduced)

1 mL

100 mL


1. Mix well according to the ratio of 4 μL protein sample to 1 μL 5× protein loading buffer per sample.

2. Metal bath or boiling water bath was heated for 3-5 min to fully denature the protein sample.

3. Cool to room temperature and add to the sample well.


1. This product contains a small amount of reducing agent DTT, has a slight pungent odor, but does not contain mercaptoethanol.Please pay attention to protection when using.

2. Make sure the product is fully rewarmed before use.

3. Please wear a lab coat and disposable gloves during operation.

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