PHONE

+86(027) 5111 3188

Share to:

G1029-100ML Fe3+ Prussian Blue Staining Solution

Specifiction: 3*100ml
Product use:Staining of trivalent iron or hemosiderin.
Storage and transportation: Store at room temperature, valid for 12 months.
ml:
  • G1029-100ML
  • Servicebio

Product Description


Product Description

Prussian blue reaction is a good method to distinctly exhibit ferric iron in tissues. Small amounts of ferric iron are found normally in spleen and bone marrow, present as ferroprotein and hemosiderin in mononuclear phagocyte or normoblast mitochondria. Any ferric ion in tissues can be differentiated out by hydrochloric acid, combines with the ferrocyanide under acid condition and results in the formation of a bright blue pigment called ferric ferrocyanide, or Prussian blue. 

Product Usage
Product Name

Code

Specifiction

Dilute Hydrochloric Acid

G1029-1

100ml

Potassium ferrocyanide solution

G1029-2

100ml

Nuclear Fast Red Solution

G1029-3

100ml


Usage:

1. Deparaffinize and rehydrate sections to distilled water.

2. Prussian blue stain:Mix equal parts of hydrochloric acid and potassium ferrocyanide immediately before use. Immerse slides in this solution for 1 hour. Wash in distilled water, 2 changes.

3. Counterstain with nuclear fast red for 1-5 min, wash in running tap water.

4. Dehydrate through 3 changes of absolute alcohol, 5 min each. Clear in xylene, 2 changes and5 min each. Mount with resinous mounting medium. 

5. Microscopy determine and analyze.


Stain Result:

Iron (ferric form) is bright blue, and nuclei is red.


Legend:


Penial tissues stained by Prussian blue. Ferric ion is blue and nucleus is red.

样例图2样例图1


Notes

1. Prussian blue stain working solution should be prepared according to the instructions immediately before use.

2. If precipitation occurs in Nuclear Fast Red Solution, filtrate it before use.

3. Use clear apparatus in the whole process, avoid metal utensils. Distilled water is better to use than tap water when washing slides and apparatus.

4. Do not use acid fix solution. Treat with chromate have adverse effect on preservation of potassium ferrocyanide.

5. Manage the staining time according to the ferric iron content.

6. Please pay attention to protect when operating.


Packing and Shipping

All of our products will be packed in standard carton.

downLoadImg

Company Information


Slice 10 Slice 11 Slice 12 Slice 13 Slice 14





Contact us

Quick Links

Product Category

Get In Touch

 5th Floor, 22 Building, Biopark, Gaoxin 2nd Road No. 388, East Lake High-Tech Developing Zone, Wuhan, Hubei, China 430079
 +86(027) 5111 3188
Copyright © 2021  Wuhan Servicebio Technology Co., Ltd.