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G1027-100ML Cartilage Tissue Alcian Blue Stain

Specifiction: 100ml
Product use:Cartilage tissue stain.
Storage and transportation: Store at room temperature, valid for 12 months.
ml:
  • G1027-100ML
  • Servicebio

Product Description


Product Description

Alcian blue is a cationic dye, stains acid mucosubstances and acetic mucins specifically. Acid mucosubstances in cytoplasm are stained blue by alcian blue. Alcian blue solution (pH=2.5) can be used for staining cartilage tissue, or combined with periodic acid-schiff staining. 

Product Usage
Product Name

Code

Specifiction

Alcian Blue Stain

G1027-100ML 100ml


Usage:

1 Deparaffinize slides and rehydrate to distilled water

2 Stain in alcian blue solution for 10-15 min, wash in running tap water for 2 min.

3 Dehydrate through 3 changes of absolute alcohol, 5 min each. Clear in 2 changes of xylene, 5 min each. Mount with resinous mounting medium.

4 Microscopy examination and analysis.


Stain Result:

For cartilage tissue, general mucins which contain carbonyl and weak acid mucous matters are stained blue, the strong acidic mucosubstances are pale or non-stain. Nucleus and background are unpigmented. 


Notes


If counterstain is necessary, Nuclear Fast Red solution can be used to stain nucleus. Combine with PAS Stain solution (G1008) is also alternative.


Packing and Shipping

All of our products will be packed in standard carton.

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