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G1015-100ML Oil Red O Staining Solution

Specifiction: 100ml
Product use:Adipose staining.
Storage and transportation: Store at room temperature, kept in dark, valid for 12 months.
ml:
  • G1015-100ML
  • Servicebio

Product Description


Product Description

Oil red O is a lipid soluble dye, showing much more soluble in the lipid than in the vehicular solvent. Based on this physical property, triglyceride in cells of tissues are specifically stained and differentiated by oil red O, presented as red. 

Product Usage
Product Name

Code

Specifiction

Oil Red O solution

G1015-100ML 100ml


Usage:

1 Fix frozen sections

Fresh frozen sections are dried to slides in air. Fix slides in formalin for 15 min, briefly wash with running water for 1-5 min, air dry.

2 Oil Red O stain

Put slides in Oil Red O staining solution to stain for 8-10 min under dark condition, wash with distilled water.

3 Differentiate

Differentiate in 75% alcohol for a few seconds, wash with distilled water.

4 Hematoxylin stain

Counterstain with hematoxylin for 3-8 min, wash with distilled water, and then quickly differentiate in 1% hydrochloric acid alcohol acid for 1 second. Wash in distilled water, bluing with 0.2% ammonia water and wash.

5 Mount

Mount in glycerine jelly.

6 Microscopy examination and analysis


Stain Result:

Lipid shows orange to sharp red and blue for nucleus.


Legend:


Hepatic tissue stained with Oil Red O. Lipid id red and nucleus is blue.

例图

Notes

1 For freshly tissue frozen section, firstly fix with formalin followed by stain. If tissue is firstly fixed with formalin before frozen section, air dry sections and then stain.

2 Be gently during the whole process. Rude action may result in lipid loss or shift.

3 The obtained staining slides cannot be preserved long-term. Carry out the microscopy examination as soon as possible after mount.

4 The glycerine jelly is solid under room temperature, and it is better preserved at 60℃ in oven. If bubbles produce during mounting, don’t press or pull apart the coverslip to avoid lipid shift. Immerse the slides in warm water to let the coverslip break off. Then remount the slides with glycerine jelly.  

Packing and Shipping

All of our products will be packed in standard carton.

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Company Information


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