Wright's Giemsa Stain is mainly used for staining blood and bone marrow smears. It is modified by the principle of Romanowsky Stain technology. The coloring process of cells is a process in which the dye penetrates into the dyed material and remains inside it. This process has both physical adsorption and chemical affinity. Due to their different chemical properties, various cells and cell components have different affinities for acid dyes (eosin) and basic dyes (methylene blue) in Wright-Giemsa staining solution. This product Wright's Giemsa staining solution is a set, where A solution contains 0.2% Wright's and 0.1% Giemsa, and B solution is a phosphate buffer solution. After the specimen smear is stained with this product, various types of cells show different colors, among which the red blood cells are light red, the white blood cell plasma particles are clear, and show the unique colors of various cells. The cytoplasmic granules of eosinophils are purplish red, the cytoplasmic granules of basophils are blue, the monocytes and lymphocytes are blue, and the nucleoplasmic staining structure is clear. To achieve the purpose of distinguishing various cell morphological characteristics.
Wright-Giemsa solution A
Wright-Giemsa solution B
1 Prepare smear sample
Cell pellet is collected by centrifugation and suspended in phosphate buffer solution. Adjust cells to a density of 106 cell/mL. Add 1 drop of the cell suspension on a microslide, then smear the suspension using another glass slide to generate a homogeneous aqueous film. Allow the smear slide dry in air.
2 Wright and Giemsa stain
Put the Wright-Giemsa solution upon the smear, in sufficient quantity to cover the entire surface, for 30 seconds.
Add 2-3 fold volume phosphate buffer solution to keep 1-3 minutes, then remove the stain solution. Rinse stained smear with distilled water until the edges show faintly pinkish red.
The film is quickly dried with dryer or allowed to dry in the air.
3 Clear with xylene for 5 minutes and mount with resin mounting medium.
4 Microscopy examination and analysis
Red blood cells are pink or flesh pink with in large amount and small volume without nuclear. Neutrophilic granulocyte shows smaller than red blood cell, with purple-blue lobulated nuclear and colorless cytoplasm. Basophil granulocyte is smaller than neutrophilic granulocyte, containing purple acidophilic granule with different size and 1-2 lobed nuclei, cytoplasm is light blue. Lymphocyte is circular with similar size with red blood cell, nuclear deep into dense. Monocyte shows the largest size with grey-blue cytoplasm, and nuclear is kidney-shaped or horseshoe-shaped in purple-blue. Blood platelet is red granule.
1、A good blood film should be in an appropriate thickness and well distributed.
2、Dry the blood film fully to prevent detachment.
3、Manage the staining time according to concentration of staining solution, temperature and cell amount.
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