PHONE

+86(027) 5111 3188

Share to:

G1002-500ML Water Soluble Eosin Y Solution

Specifiction: 100ml/500ml
Product use:Cytoplasmic staining.
Storage and transportation: Store at room temperature, valid for 6 months.
ml:
  • G1002-500ML
  • Servicebio

Product Description


Product Description

Eosin is an acid dye used histologically to stain cytoplasm. The basic principle is that eosin can be dissociated into negatively charged anions in water, which can combine with the cationic amino groups of proteins in the cytoplasm to make the cytoplasm appear red. The molecular weight of eosin (water soluble) used in this product is 691.85, and the concentration is 0.5%. It is usually used in combination with hematoxylin dye (G1004) to stain tissues or cells and display cell structure. After staining, the cytoplasm appears in varying shades of pink to peach. Compared with G1001 eosin dye (alcohol solution), tissue or cells stained with this product need to undergo gradient ethanol dehydration before xylene transparent and sealed, tissue or cytoplasm staining is lighter than G1001.

Product Usage
Product Name

Code

Specifiction

Eosin Y(Water Soluble)

G1002-100ML 100ml

G1002-500ML

500ml


Usage:

1) Deparaffinize and rehydrate to distilled water: Deparaffinize sections in 2 changes of xylene, 10 minutes each. Dehydrate in 95% alcohol and 80% alcohol respectively, 1 minute each. Wash in running tap water for 1 minute to 2 minutes

2) Stain with hematoxylin solution for 5-10 minutes, wash in tap water for 1 minute to 2 minutes

3) Differentiate in 1% hydrochloric acid alcohol for 30 seconds. Wash in running tap water for 5-10 minutes for bluing, or rinse in 0.2% ammonia water for 30 seconds and wash in running tap water for 1 minute to 2 minutes for bluing.

4) Counterstain in Eosin Y solution (water) for 30 seconds to 1 minute. Wash briefly in distilled water.

5) Dehydrate successively in 80% alcohol, 90% alcohol, and 2 changes in absolute alcohol, 5 seconds each. Clear in 2 changes of xylene, 5 minutes each. Mount with resin mounting medium.

6) Microscopy examination and analysis.



Staining result:

Nuclei should be blue, cytoplasm pink to red.


Notes

1. Eosin dye (water soluble) can be used repeatedly for several times. When the tissue staining is obviously shallow, it is recommended to replace a new dye.

2. Please wear a lab coat and disposable gloves during operation.


Packing and Shipping

All of our products will be packed in standard carton.

downLoadImg

Company Information


Slice 10 Slice 11 Slice 12 Slice 13 Slice 14





Contact us

Quick Links

Product Category

Get In Touch

 5th Floor, 22 Building, Biopark, Gaoxin 2nd Road No. 388, East Lake High-Tech Developing Zone, Wuhan, Hubei, China 430079
 +86(027) 5111 3188
Copyright © 2021  Wuhan Servicebio Technology Co., Ltd.