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DNase I (Deoxyribonuclease I, deoxyribonuclease I) is an endonuclease that can degrade single-stranded and double-stranded DNA into 5'-phosphate, 3'-hydroxy-terminal single deoxynucleotides or oligonucleotides Acid; the activity of DNase I depends on calcium ions and can be activated by magnesium ions and divalent manganese ions; in the presence of magnesium ions, DNase I can randomly cut any position of double-stranded DNA; in the presence of divalent manganese ions, DNase I can cut double strands of DNA at the same site to form blunt ends or sticky ends with 1-2 nucleotides protruding.
Main purpose: prepare RNA samples without DNA
Removal of genomic DNA residues in RNA samples before RT-PCR reaction
In vitro T7, T3, SP6 and other RNA Polymerases catalyzed the removal of DNA template in the post-RNA transcription system
Partial shearing of genomic DNA in the apoptosis Tunel test serves as a positive control for the experiment, etc.
Definition of enzyme activity: Incubate at 37°C for 10 min to define the amount of enzyme required for the complete degradation of 1 µg of pBR322 vector DNA as one unit of enzyme activity.
Inactivation or inhibition: After adding EDTA, heating at 65°C for 10 min can fully inactivate DNase I, and phenol LF extraction can also inactivate DNase I
Purity: SDS-PAGE detection purity ≥95%, no RNase, 1 U/μL
Enzyme storage buffer: 10 mM Tris-HCl, 2 mM CaCl2, 50% Glycerol, pH 7.6.
10×DNase I Reaction Buffer：100 mM Tris-HCl，25 mM MgCl2，5 mM CaCl2，pH 7.6。
Storage and transportation
Transport with wet ice; storage at -20°C, valid for 12 months.