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DMEM/F-12, HEPES without L-glutamine Cell Culture Media

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Cat. NO.: G4613-500ML

Volume: 500mL

Feature: HEPES without L-glutamine

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  • G4613-500ML
  • Servicebio

Product Introduction


DMEM/F-12 medium is modified by mixing DMEM and Ham’s F-12 medium at a ratio of 1:1. The modified DMEM/F-12 medium is richer in nutrients and contains more trace elements. It is widely used in the cultivation of a variety of mammalian cells, and also suitable for the cultivation and cloning of mammalian cells with low serum content. Density cultivation. DMEM/F-12 is also used as a basic medium for the development of serum-free medium. This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture. It does not contain protein or growth factors. It is necessary to add 5-10% serum or serum-free additives according to the cell type.


This product is sterilized through a 0.1 μm filter membrane, pH 7.0-7.4, containing 17.5 mM D-glucose, adding 0.5 mM sodium pyruvate, containing phenol red indicator, containing 15 mM HEPES buffer system, without L-glutamine .


HEPES is a commonly used biological buffer, which can keep the culture medium in a constant pH range for a long time and effectively prevent large fluctuations in the pH of the culture fluid from adversely affecting cell growth.


Storage and transportation


Transport at room temperature; store at 2-8°C, valid for 12 months.



Precautions


1. This product has been filtered and sterilized. When using it, you should pay attention to aseptic operation to avoid contamination;


2. In order to maintain the best results, please do not freeze-thaw.

G4613-500ML DMEM F-12 白底-1

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