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Permeabilization solution is used to treat cell membrane before staining or IHC. Permeabilization solution can make the complete cell membrane produce small holes to facilitate the entry of antibodies into the cell. Triton X-100 is the main component of the Permeabilization solution produced by our company.
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Stored at 4ºC, valid for 6 months.
The slices were dried slightly and then dripped Permeabilization solution into the circle to cover the tissue, incubated at room temperature for 20-30min, then placed in the PBS (pH 7.4) and washed 3 times on the pendulum shaker for 5min each time, then to the next step.
1. For cell samples, if you need to use Triton X-100 less than 0.5%, you can use PBS buffer to dilute this product.
2. For samples that are difficult to penetrate, the processing time can be extended as appropriate.
3. Please wear lab coat and disposable gloves during operation.