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Anti -beta Actin Mouse MAb Western IHC IF Loading Control Antibody

Cat. No. GB12001

Brand:Servicebio

Specification: 100uL

  • GB12001
  • Servicebio
  • Servicebio

Product Description

Product Information


Description

beta Actin mouse monoclonal

Protein full name

Actin, cytoplasmic 1

Synonyms

ACTB, BRWS1, PS1TP5BP1, Beta-actin, actin beta, Beta Cytoskeletal Actin, β-Actin

Immunogen

KLH conjugated Synthetic peptide

corresponding to Mouse β-Actin

Isotype

IgG

Predicted MW.

42 kDa

Observed MW.

45 kDa

Purity

Affinity purification

Subcellular location

Cytoplasm

Uniprot ID

P60709, P60710, P60711



Applications


Applications

Species

Dilution

Positive Tissue

WB

Human, Rat, Mouse

1: 1000-1: 2000

kidney, brain, spleen

IHC

Human, Rat, Mouse

1: 400-1: 2000

para-cancer colon, lung, stomach, liver cancer

IF

Human, Rat, Mouse

1: 400-1: 2000

para-cancer colon, lung, stomach, liver cancer, 



Background



Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.




Images



Western blot analysis of β-Actin(GB12001) 

at dilution of 1: 1000

Western blot analysis of β-Actin(GB12001) 

at dilution of 1: 1000

Immunohistochemistry analysis of paraffin-embedded human liver cancer using β-Actin(GB12001) at dilution of 1:400


Immunohistochemistry analysis of paraffin-embedded human para-cancer colon using β-Actin(GB12001) at dilution of 1:400

Immunohistochemistry analysis of paraffin-embedded mouse stomach using 

β-Actin(GB12001) at dilution of 1:400

Immunohistochemistry analysis of paraffin-embedded rat liver using 

β-Actin(GB12001) at dilution of 1:400

Immunohistochemistry analysis of paraffin-embedded rat kidney using 

β-Actin(GB12001) at dilution of 1:400




Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).



Publications:


Title

Journal

Pubmed ID

Application

1

Time-Restricted Feeding Reduces the Detrimental Effects of a High-Fat Diet, Possibly by Modulating the Circadian Rhythm of Hepatic Lipid Metabolism and Gut Microbiota.

Frontiers in Nutrition

33425971

WB

2

Linalool inhibits 22Rv1 prostate cancer cell proliferation and induces apoptosis.

Oncology Letters

33029205

WB

3

TRIM3 inhibits P53 signaling in breast cancer cells.

Cancer Cell International

33292295

WB

4

CAB39 Promotes the Proliferation of Nasopharyngeal Carcinoma CNE-1 Cells via Up-Regulating p-JNK.

Cancer Management and Research

33177871

WB

5

MicroRNA 23a 5p mediates the proliferation and differentiation of C2C12 myoblasts.

Molecular Medicine Reports

32901860

WB

6

NLRC5 Inhibits Inflammation of Secretory Phase Ectopic Endometrial Stromal Cells by Up-Regulating Autophagy in Ovarian Endometriosis.

Frontiers in Pharmacology

33013364

WB

7

RACO-1 modulates Hippo signalling in oesophageal squamous cell carcinoma.

Journal of Cellular and Molecular Medicine

32896069

WB

8

CDE-1 suppresses the production of risiRNA by coupling polyuridylation and degradation of rRNA.

BMC Biology

32887607

WB

9

Synergistic Effects of Ginkgolide B and Protocatechuic Acid on the Treatment of Parkinson's Disease.

Molecules (Basel, Switzerland)

32878312

WB

10

Effect of Moxibustion on the Intestinal Flora of Rats with Knee Osteoarthritis Induced by Monosodium Iodoacetate.

Evidence-based Complementary and Alternative Medicine : eCAM

32714401

WB


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