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Alizarin Red S Stain for Dyeing A Small Amount of Calcium Salt Deposits

  • G1038-100ML

Product Description

Principle

Alizarin red S, an anthraquinone derivative, has a chemical name of Alizarin sulfonic acid sodium salt. It can form orange chelate complexes with different calcium, including calcium carbonate and tricalcium phosphate. This product may be used to identify deposits of calcium salt in tissues, pathological calcified sediment such as in tuberculosis caseous necrosis foci, arterial wall of atherosclerosis, dead parasitic ovum, and other calcified matters.

 

Package content

Code

Name

Size

G1038

Alizarin red S soluiton

100ml

 

Preservation

Valid in 12 months at room temperature.

 

Procedure   

1、Deparaffinize and hydrate sections to distilled water.

2Slides stain in Alizarin red S for 5-10 min, wash in tap water. Dry slides in oven. 

3、Clear in xylene for 5 min and mount with resin mounting medium.

4、Microscopy determination and analysis.

Results 

Calcium deposits are deep red in light red or colorless background.

 

Notes
1、 manage the staining time by microscopy observation. Too long for staining may result in color diffusion.

2、If any droplet on slides after clear in xylene, dry slides and rinse in butanol for 10sand then re-clear in xylene. 

 

Example

 

Human tumor tissue stained with Alizarin red S. Calcium salt are shown in red.

图片5Slice 13Slice 10Slice 12 


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