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TBST buffer, namely TBS with Tween-20, is an isotonic buffer salt solution commonly used in biology. Tris-HCl constitutes a stable pH buffer system, NaCl provides isotonic conditions, and Tween-20 as a detergent can increase buffering The elution capacity of the liquid. It can be used in Western Blot experiments to wash off non-specifically bound antibodies and other reagents on the membrane, as well as in the preparation of blocking solutions, the preparation of primary or secondary antibodies, and the preparation of primary or secondary antibodies in experiments such as immunofluorescence and immunohistochemistry. Washing etc. to reduce the background and enhance the signal-to-noise ratio. This product is a 10 times concentrated solution, the main components are 100 mM Tris, 1.5 M NaCl, 1% Tween-20, it needs to be diluted 10 times before use.
Product Item No.
Store at 4°C, valid for 12 months. It can be stored for a long time at -20°C
Take 100 mL of TBST and mix thoroughly with 900 mL deionized water or distilled water to obtain a 1×TBST working solution containing 10 mM Tris, 150 mM NaCl, 0.1% Tween-20, and pH 7.4-7.6@25°C.
1. After this product is diluted into 1× working solution, it should be stored at 4°C and used up within one month. If stored at room temperature, it is recommended to use it within a week.
2. If the product crystallizes out, please place it in a 37°C water bath until it is completely dissolved, which will not affect the usage.
3. Please wear lab coat and disposable gloves during operation.