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20x Tris-EDTA Repair Buffer Solution(pH 8.0) Antigen Retrieval Buffer

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Cat. NO.:G1206-250ML
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  • G1206-250ML
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20×Tris-EDTA Antigen Retrieval Solution(pH 8.0)

G1206-250ML

250 mL

G1206-250ML

Description/Introduction

Immobilization of cells or tissues with paraformaldehyde, formaldehyde or other aldehyde reagents results in cross-linking of proteins, obscuring the antigenic site of the sample, leading to diminished staining signals during immunostaining and even false positive staining results.The protein crosslinking caused by aldehydes is reversible. Under certain conditions, such as high temperature or protease hydrolysis, the original conformation of the protein can be restored. This process is antigen retrieval. Antigen repair is a key step in the process of immunohistochemistry. There are many kinds of antigen repair methods. Different methods can be selected according to the characteristics of tissue types. Some are suitable for thermal repair and some are suitable for enzymatic repair. There are also many different choices to pH value of antigen repair solution due to the characteristics of antigen. The company provides a variety of antigen repair solutions with different pH values, which can meet the needs of a variety of antigen repair.

This product 20×Tris-EDTA repair solution (pH 8.0) is a concentrated repair solution. The main component of the product is 20 mM EDTA. After 20 times dilution, a solution contained 1.0 mM EDTA with pH 8.0 can be obtained.


Storage and Handling Conditions

Normal temperature storage and transportation; Valid for 12 months

Assay Protocol

Mix 10 mL 20×Tris-EDTA antigen repair solution(pH 8.0) with 190 mL distilled water to obtain 1× antigen repair solution contained 1.0 mM EDTA.with pH 8.0.

Note:

1. This product is a concentrated solution and needs to be diluted before use. It is recommended to use up the diluted buffer on the same day and store it briefly at 4℃.

2. Please select the appropriate antigen repair solution according to the experimental needs and antigen characteristics. If acidic or weakly alkaline repair solution is required, refer to G1202 and G1203.

3. Please wear lab coat and disposable gloves to operate.

For Research Use Only!

1. This product is a concentrated solution and needs to be diluted before use. It is recommended that the diluted buffer be used up on the same day and can be stored briefly at 4°C.


2. Please select the appropriate antigen retrieval solution according to the experimental requirements and antigen characteristics. For acidic or alkaline repair fluids, please refer to G1202 and G1203.


3. Please wear lab coat and disposable gloves during operation.

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