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10% SDS-Page Color Gel Ultra-Fast Preparation Kit Green

  • G2062-50T

Product Description

Product description

This kit provides a simple and fast 10% SDS-PAGE color (green) gel preparation method. Users only need to prepare their own gel-making equipment to prepare the gel, which greatly simplifies the gel-making process. The upper gel (concentrated gel) reagent provided in the kit can prepare a colored (green) upper gel. The green staining kit can stably exist in the upper gel (concentrated gel, accumulated gel), and will not enter the lower gel with electrophoresis ( Separating gel), so the addition of the green dye can make the upper gel protein spotting hole clearly appear, it is easy to judge the margin of the sample hole or whether it is distorted or damaged, and it is convenient to add the protein sample to the spotting hole accurately. The staining kit will not affect the electrophoresis and staining effect. After the electrophoresis is completed, it is easy to identify and remove the upper layer gel, and does not affect subsequent Western Blot and other experiments. The specific number of gel blocks is related to the thickness of the gel and the size of the gel.

Storage and transportation 
Wet ice Transportation ;
Stored at 4°C and away from light, valid for one year;

Composition

Component G2062-50T
Upper gel solution A 50mL
Upper gel solution B (green) 50mL
10% lower gel solution A 125mL
10% lower gel solution B 125mL
AP (Ammonium Persulfate) 1g(Powder)
User manual 1 pc


Steps

  1. According to the molecular weight of the target protein, select the appropriate concentration of SDS-PAGE lower gel (separation gel), please refer to the following table:

SDS-PAGE separation gel concentration Best separation range (kDa)
6% 50-250
8% 30-130
10% 20-100
12% 10-60
15% <40


2.Prepare 10% AP solution: Get 0.1 g of AP (ammonium persulfate) powder and dissolve it completely with 1 mL of water. The solution is valid for 1-2 weeks when stored at 4°C, and valid for 3 months when stored at -20°C.

3.According to the experimental requirements, mix solution A and B in equal proportions, add an appropriate amount of 10% AP solution, and prepare the lower gel solution and the upper layer gel solution respectively. For glass plates of different specifications and different thicknesses, the preparation volume of the upper gel and the lower gel solution can be adjusted in equal proportions. Taking the commonly used 8.3 cm×7.3 cm gel plate as an example, the recommended preparation system as follows:
 

Group Component Single piece of gel (1.0 mm thickness) Two pieces of gel (1.0 mm thickness)



Lower gel solution
10% lower gel solution A 2.5mL 5mL
10% lower gel solution B 2.5mL 5mL
10% AP solution 50μL 100mL



Upper gel solution
Upper gel solution A 1mL 2mL
Upper gel solution B (green) 1mL 2mL
10% AP solution 20μL 40μL


Note: According to different manufacturers and glass plates of different thicknesses, the volume of the upper gel and lower gel solution can be adjusted in equal proportions. Add 10% AP (100 times dilution) to the solution and mix it thoroughly.

4. Prepare the gel tool and pour the prepared lower layer gel solution, and immediately add the prepared upper layer gel solution slowly and evenly (distilled water can also be used to seal the lower layer gel liquid surface, 15-30 minutes later until the lower layer gel solidifies, add the prepared upper layer gel solution after it is full), insert the comb, and wait for it to solidify (about 15-30 min) before usage.

5. If the prepared gel cannot be used on the same day, it can be stored at 4°C for 1-2 days before usage.

Precautions

1. There is monomer acryamide in the premix, which is harmful to the human body. Please take protective measures during operation.

2. In order to ensure that the sample enters the separation gel at the same time, when directly pouring the top gel, the prepared top gel solution should be added slowly and evenly.

3. Pour the gel as soon as possible after adding AP, do not leave it for a long time.

4 . The solidification of the gel has a significant positive correlation with the temperature. Under the same conditions, the higher the temperature, the faster the solidification rate.
 

5.This product has been added with an appropriate amount of TEMED substitutes. If you need to further accelerate the gel speed, you can add an appropriate amount of TEMED as needed before the gel is prepared, and at the same time increase the AP usage by 0.5 times.

 

6.The company also provides other color (red, yellow, blue) top gel solutions to distinguish different samples and different gel electrophoresis.

 

7.The running buffer is suitable for the Tris-Glycine buffer system (recommended our product: G2018-1 L running buffer).

G2062-50T-=-

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