$ 7.5 / slide
|sirius red staining ovary collagen fiber red background yellow||heart sirius red||heart sirius red|
|sirius red staining kidney||sirius red staining liver||sirius red staining|
1.Deparaffinage and hydrate to water: following next steps, put paraffin sliced into XyleneⅠ20 mins,and XyleneⅡ 20 mins, anhydrous ethanol 5mins, and 75% anhydrous ethanol 5 mins, at last tap water is washed.
2.The sirius Red Stain：the slices were cut into Sirius red stain solution 8-10 mins, two or three tanks dehydrated with anhydrous ethanol.
3.Dehydrate seal: Slice and put in clean xylene transparent 5 mins,Glycerol Jelly seal.
4.Microscopic examination and image collection analysis.
Under normal light microscope, the collagen fibers were red and the others were yellowish. Under polarized light microscopy, the I-type fiber are closely aligned, showing a strong birefringence, with yellow or red fibers. And Ⅱ-type fiber, showing weak double refraction, a greenish fine fiber. Ⅳ type fiber, showing weak double refraction basilemma, pale yellow.