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Home > Images > Safranin O-Fast Green Staining (bone tissue)

Safranin O-Fast Green Staining (bone tissue)

$ 7.5 / slide


上传6-R-L safranin fast green_16.2x 1缩略图.jpg上传6-R-L safranin fast green_16.2x缩略图.jpg上传COA-3 safranin fast green_18.6x缩略图.jpg
Safranin O-Fast Green stainingSafranin O-Fast Green stainingSafranin O-Fast Green staining
上传safranin fast green staining bone 40-1缩略图.jpg上传safranin fast green staining bone 100-1缩略图.jpg上传safranin fast green staining bone 100-2缩略图.jpg
Safranin O-Fast Green stainingSafranin O-Fast Green stainingSafranin O-Fast Green staining
上传safranin fast green staining fetal rat leg bone 40-1缩略图.jpg上传tooth masson_3.5x缩略图.jpg
Safranin O-Fast Green stainingSafranin O-Fast Green staining


Protocol 

1.Deparafiinize and rehydrate to distilled water.


2.Staining with Fast-Green solution 5-10 mins, wash to remove the excess stain solution until cartilage is colorless. Rinse in hydrochloric acid alcohol slight to differentiate, wash in tap water. 

 

3.Staining in Safranin O solution for 13-30 s, rapid dehydration by 3 cylinders absolute alcohol.


4.Clear in xylene 5 mins, mount with resin mounting medium.


5.Microscopy determination and analysis.


Results

Cartilage is red or orange in green background.


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