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Home > Images > Safranin O-Fast Green Staining (plant)

Safranin O-Fast Green Staining (plant)

$ 7.5 / slide


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safranin fast green
safranin fast green staining pea pod  lignin red, cellulose green 100 times
safranin fast green
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上传safranin fast green staining stem lignin red, cellulose green, 200 times缩略图.jpg上传safranin fast green staining seed  lignin red, cellulose green 100 times缩略图.jpg
safranin fast green staining stem lignin red, cellulose green, 200 timessafranin fast green staining seed  lignin red, cellulose green 100 times


Protocol

1.Deparaffinize and rehydrate sections to distilled water.

2.Safranin O stain: Immerse slides in Safranin O solution for 1-2 h, rinse in tap water to remove excess dye.

3.Dehydrate successively in 50%, 70%, 80% alcohol, respectively, each 3-8 s 

4.Fast green stain: Immerse slides in fast green solution for 30-60 s, dehydrate 3 changes in absolute alcohol.

5.Clear and mount: Clear in xylene for 5 min, mount with resin mounting medium.

6.Microscopy determination and analysis. 


Results

Lignified cells are red, while the fibrous cells are green.


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