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Home > Images > Toluidine Blue Staining

Toluidine Blue Staining

$ 7.5 / slide


上传W10-2 Toluidine blue Staining _1.2x缩略图.jpg上传Toluidine blue Staining Root Cell wall is Blue 100倍缩略图.jpg上传Toluidine blue Staining Germ  Cell wall is Blue 100倍缩略图.jpg
Toluidine blue StainingToluidine blue Staining RootToluidine blue Staining Germ
上传11 Toluidine blue Staining Cartilage Purple red 40-1缩略图.jpg上传Toluidine blue Staining Cartilage 100-1缩略图.jpg上传Toluidine blue Staining -2 Cartilage is purple red,Background is ble 100倍缩略图.jpg
Toluidine blue Staining Cartilage Purple redToluidine blue Staining Cartilage 
Toluidine blue Staining -2 Cartilage is purple red,Background is ble
上传Spinal cord Nissl Body_29.0x缩略图.jpg上传Toluidine blue Staining Hippocampus Nissl Body 200-1缩略图.jpg上传10 Toluidine blue Staining   Muscular Layer Mast cell Purple Red 400-2缩略图.jpg
Spinal cord Nissl BodyToluidine blue Staining Hippocampus Nissl Body
Toluidine blue Staining   Muscular Layer Mast cell Purple Red


Protocol

1.Deparaffinize and hydrate sections to distilled water.


2. Stain in toluidine blue solution

 For plant tissue slides, stain in toluidine blue solution for 2-5 min, wash in tap water. Observe by microscopy to check the staining extent. If necessary, differentiate properly according to the staining extent, wash in tap water. Dry slides in oven.


For animal tissue slides, stain in toluidine blue solution for 5 min, wash in tap water. Differentiate in 1% glacial acetic acid slightly and stop by washing in tap water. Manage the differentiate extent by microscopy observation. Dry slides in oven. 


3. Clear and mount. Clear in xylene for 5 min and mount with resin mounting medium.


4. Microscopy determination and analysis.


Results

1.Plant tissue: cell wall of lignified tissue is blue-green, while the fibrous tissue is purple-blue.

2.animal tissue: the Nissl body in brain tissue is deep blue, background is light blue; for bone tissue, cartilage is purple-blue, background is light blue; for intestinal tissue, the mast cells are purple-red, nucleus and background are light blue.


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