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Home > Images > Masson Trichrome Staining

Masson Trichrome Staining

$ 7.5 / slide


上传1 masson染色 心梗 40-1缩略图.jpg

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上传1 masson 皮肤损伤 40-1缩略图.jpg

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Masson skin damageMasson hepatic parasite


Protocol

1.Deparaffinage and rehydrate by immersing the slides through the following:

1.1. Xylene: two times washes for 20 minutes each

1.2. 100% alcohol: two times washes for 5 minutes each

1.3. 75% alcohol: wash for 5 minutes

1.4. Wash briefly in tap water


2.Potassium dichromate stain

Rinse slides in Potassium dichromate mordant solution overnight, wash in tap water quickly.


3. Iron hematoxylin stain

Mix up the Weigert hematoxylin iron solution A with solution B by equivalent volume to generate iron hematoxylin solution. Rinse the slides in this iron hematoxylin solution for 3 minutes, wash in tap water. Then differentiate in 1% acid alcohol for 30 seconds, wash in running tap water.


4.Acid ponceau fuchsin stain

Stain in acid ponceau fuchsin solution for 3-5 minutes, rinse in tap water to wash.


5.Phosphomolybdic acid stain

 Immerse slides in phosphomolybdic acid solution for 1-3 minutes. Do not wash in water.


6.Aniline blue stain

Stain the slides with aniline blue solution for 3-6 minutes.


7.Differentiate

Differentiate slides in 1% glacial acetic acid solution, dehydrate in 3 changes of absolute alcohol, 5 minutes each.


8.Clearing and mounting

Clear in xylene for 5 minutes, mount with resin mounting medium.


9.Microscopy examination and analysis


Results

Collagen fiber should be blue; muscle fiber, cellulose and red blood cells should be red. 


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