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Home > Images > HE Staining

HE Staining

$ 3 / slide


HE染色 脑 冠状切面整体观缩略图.jpg


附睾_1.8x缩略图.jpg


HE染色 斑马鱼 矢状切面缩略图.jpg
HE Brain Coronal section
HE Epididymis
HE Sagittal section of zebra fish
HE染色 肌肉 肌纤维横断面缩略图.jpg
皮肤-1缩略图.jpg
脾-1_5.2x缩略图.jpg
HE  Muscle  Cross section of muscle fibers
HE Skin
HE Spleen
肾_1.0x缩略图.jpg
心 纵切_1.0x缩略图.jpg
子宫 纵_2.5x缩略图.jpg
HE Kidney
HE Heart Cross section
HE Uterus Cross section


Protocol

1.Deparaffinize sections, 2 changes of xylene, 20 minutes each. Dehydrate in 2 changes of absolute alcohol, 5 minutes each. Rinse 75% alcohol for 5 minutes. Wash briefly in tap water.

2.Stain in hematoxylin solution for 3-5 minutes. Differentiate in acid alcohol Then bluing in ammonia water, wash in running tap water for 5 minutes.

3.Dehydrate through 85% alcohol and 95% alcohol respectively. Counterstain in Eosin Y solution (alcohol) for 5 minutes.

4.Dehydrate in 3 changes of absolute alcohol, 5 minutes each. Clear in 2 changes of xylene, 5 minutes each. Mount with resin mounting medium.

5.Microscopy examination and analysis


Results

Nuclei should be blue, cytoplasm pink red. The nucleoplasm is clear and contrasting.


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