$ 3 / slide
|HE Brain Coronal section||HE Epididymis||HE Sagittal section of zebra fish|
|HE Muscle Cross section of muscle fibers||HE Skin||HE Spleen|
|HE Kidney||HE Heart Cross section||HE Uterus Cross section|
1.Deparaffinize sections, 2 changes of xylene, 20 minutes each. Dehydrate in 2 changes of absolute alcohol, 5 minutes each. Rinse 75% alcohol for 5 minutes. Wash briefly in tap water.
2.Stain in hematoxylin solution for 3-5 minutes. Differentiate in acid alcohol Then bluing in ammonia water, wash in running tap water for 5 minutes.
3.Dehydrate through 85% alcohol and 95% alcohol respectively. Counterstain in Eosin Y solution (alcohol) for 5 minutes.
4.Dehydrate in 3 changes of absolute alcohol, 5 minutes each. Clear in 2 changes of xylene, 5 minutes each. Mount with resin mounting medium.
5.Microscopy examination and analysis
Nuclei should be blue, cytoplasm pink red. The nucleoplasm is clear and contrasting.